Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
نویسندگان
چکیده
We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.
منابع مشابه
Reflex™: a novel method to sequence barcoded long-range PCR products in a pooled population of hundreds of DNA samples
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عنوان ژورنال:
دوره 41 شماره
صفحات -
تاریخ انتشار 2013